Areas C. elegans Neurotransmitters Exocytosis Ultrafast Endocytosis Clocks Axons Transposons Attraction Microscopy
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Featured Publications
CaV1 and CaV2 calcium channels mediate the release of distinct pools of synaptic vesicles
CaV1 and CaV2 calcium channels mediate the release of distinct pools of synaptic vesicles

Activation of voltage-gated calcium channels at presynaptic terminals leads to local increases in calcium and the fusion of synaptic vesicles containing neurotransmitter. We demonstrate that synaptic vesicle pools are separated into two pools at C. elegans presynapses. At the center of the active zone, vesicles are tethered to the CaV2 neuronal-type calcium channel. Laterally, vesicles are tethered to the CaV1 muscle-type calcium channels and internal calcium stores by the ryanodine receptor. Vesicles are recruited to these fusion sites by different isoforms of the UNC-13 protein.

Interspecies complementation identifies a pathway to assemble SNAREs
Interspecies complementation identifies a pathway to assemble SNAREs

To understand the functional interactions within the core SNARE machinery we adopted an ‘interspecies complementation’ approach in C. elegans. Substitutions of individual SNAREs and Unc18 proteins with those from yeast fails to rescue fusion. However, synaptic transmission could be restored in worm-yeast chimeras when two key interfaces were present: a syntaxin Habc-Unc18 contact site and an UNC-18-SNARE motif contact site. Finally, we demonstrate that the SNARE and UNC-18 machinery in the nematode C. elegans can be replaced by yeast proteins and still carry out synaptic transmission, pointing to the deep evolutionary conservation of these two interfaces.

Synaptic vesicles transiently dock to refill release sites.
Synaptic vesicles transiently dock to refill release sites.

To characterize the ultrastructure of vesicle docking and fusion at active zones, we developed a method called ‘zap-and-freeze’ to trigger single action potentials by electrical stimulation followed by high-pressure freezing at defined time points. Using this approach, we first characterized the spatial and temporal organization of fusion sites following a single action potential. We observed that during synchronous release, multiple vesicles can fuse per action potential within the same active zone, even in physiological extra- cellular [Ca2+]. Fusions during synchronous release occur through- out the active zone, but are concentrated at the center of the active zone during asynchronous release.

Casein Kinase 1d Stabilizes Mature Axons by Inhibiting Transcription Termination of Ankyrin
Casein Kinase 1d Stabilizes Mature Axons by Inhibiting Transcription Termination of Ankyrin

LaBella et al.

Developmental Cell 2020

During early development growth cones sprout from neurons and lay down an axon to the neuron’s synaptic targets. After axon outgrowth and synapse formation, the nervous system transitions to a stable architecture that lasts the lifetime of the animal. During this transition, synapses must send a signal to the cell body to reprogram the nucleus. In C. elegans, this transition is mediated by casein kinase 1δ (CK1δ), which modifies the behavior of RNA polymerase II. RNA polymerase has a long C-terminal domain composed of 20-50 repeats of a 7 amino acid sequence. Phosphorylation of serine-5 in this sequence marks actively transcribing polymerase, and removal of this phosphate by the termination factor SSUP-72 initiates transcription termination. CK1δ phosphorylates and inhibits SSUP-72, thereby inhibiting termination. The target of this switch is the gene encoding the cytoskeleton protein Ankyrin. CK1δ activity switches expression from the short to giant isoform of this protein.

 

Mueller, BD, Merrill SA, von Diezmann L, Jorgensen EM. Using Localization Microscopy to Quantify Calcium Channels at Presynaptic Boutons. Bio-protocol 2024 14(16): e5049. DOI: 10.21769/BioProtoc.5049.

Fernando, S.I., Martineau, J.T., Hobson, R.J., Vu, T.N., Baker, B., Mueller, B.D., Menon, R., Jorgensen, E.M., and Gerton, J.M. Simultaneous spectral differentiation of multiple fluorophores in super-resolution imaging using a glass phase plate. Opt. Express, 2023, OE 31, 33565–33581. https://doi.org/10.1364/OE.499929.

Palfreyman MT, West SE, Jorgensen EM. SNARE Proteins in Synaptic Vesicle Fusion. Adv Neurobiol. 2023;33:63-118. doi: 10.1007/978-3-031-34229-5_4. PMID: 37615864.

Mueller BD, Merrill SA, Watanabe S, Liu P, Niu L, Singh A, Maldonado-Catala P, Cherry A, Rich MS, Silva M, Maricq AV, Wang ZW, Jorgensen EM. CaV1 and CaV2 calcium channels mediate the release of distinct pools of synaptic vesicles. Elife. 2023 Feb 23;12:e81407. doi: 10.7554/eLife.81407. PMID: 36820519.

Parra-Rivas LA, Palfreyman MT, Vu TN, Jorgensen EM. Interspecies complementation identifies a pathway to assemble SNAREs. iScience. 2022 Jun 2;25(7):104506. doi: 10.1016/j.isci.2022.104506. PMID: 35754735; PMCID: PMC9213704.

Davis MW, Jorgensen EM. ApE, A Plasmid Editor: A Freely Available DNA Manipulation and Visualization Program. Front Bioinform. 2022 Feb 4;2:818619. doi: 10.3389/fbinf.2022.818619. PMID: 36304290; PMCID: PMC9580900.

Guo R, Nelson S, Regier M, Davis MW, Jorgensen EM, Shepherd J, Menon R. Scan-less machine-learning-enabled incoherent microscopy for minimally-invasive deep-brain imaging. Opt Express. 2022 Jan 17;30(2):1546-1554. doi: 10.1364/OE.446241. PMID: 35209312; PMCID: PMC8970698.

Rich MS, Nix P, Jorgensen EM. The C. elegans mapping locus rol-9 is encoded by a gain-of-function mutation in mlt-11. MicroPubl Biol. 2022 Jan 5;2022:10.17912/micropub.biology.000506. doi: 10.17912/micropub.biology.000506. PMID: 35005481; PMCID: PMC8733821.

Schwartz ML, Davis MW, Rich MS, Jorgensen EM (2021) High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. PLoS Genet 17(11): e1009755. https://doi.org/10.1371/journal.pgen.1009755

Xiao T, Yan A, Huang JD, Jorgensen EM, Shah NP. Comparative Peptidomic and Metatranscriptomic Analyses Reveal Improved Gamma-Amino Butyric Acid Production Machinery in Levilactobacillus brevis Strain NPS-QW 145 Cocultured with Streptococcus thermophilus Strain ASCC1275 during Milk Fermentation. Appl Environ Microbiol. 2020 Dec 17;87(1):e01985-20. doi: 10.1128/AEM.01985-20. PMID: 33067198; PMCID: PMC7755244.

Watanabe, S., Davis, M.W., Kusick, G.F., Iwasa, J., and Jorgensen, E.M. (2020). SynapsEM: Computer-Assisted Synapse Morphometry. Frontiers in Synaptic Neuroscience 12, 55.

Kusick, G.F., Chin, M., Raychaudhuri, S., Lippmann, K., Adula, K.P., Hujber, E.J., Vu, T., Davis, M.W., Jorgensen, E.M., and Watanabe, S. 2020. Synaptic vesicles transiently dock to refill release sites. Nature Neuroscience 1–10.

 LaBella M.L., Hujber E.J., Moore K.A., Rawson R.L., Merrill S.A., Allaire P.D., Ailion M., Hollien J., Bastiani M.J., Jorgensen E.M. 2020. Casein Kinase 1δ Stabilizes Mature Axons by Inhibiting Transcription Termination of Ankyrin. Developmental Cell. 52(1):88-103.e6.

Appadurai, D., Gay, L., Moharir, A., Lang, M.J., Duncan, M.C., Schmidt, O., Teis, D., Vu, T.N., Silva, M., Jorgensen, E.M., et al. 2019. Plasma membrane tension regulates eisosome structure and function. MBoC 31, 287–303.

Watanabe, S., L.E. Mamer, S. Raychaudhuri, J. Eisen, T. Trimbuch, B. Söhl-Kielczynski, P. Fenske, I. Milosevic, C. Rosenmund, E.M. Jorgensen. 2018. Synaptojanin and Endophilin Mediate Neck Formation during Ultrafast Endocytosis. Neuron, 98:1184-1187.

Chen, B., P. Liu, E. J. Hujber, Y. Li, E.M. Jorgensen, Z.W. Wang. 2017. AIP limits neurotransmitter release by inhibiting calcium bursts from the ryanodine receptor, Nature Communications, 8:1380.

Palfreyman, M.P., E. M. Jorgensen. 2017. Unc13 Aligns SNAREs and Superprimes Synaptic Vesicles. Neuron 85:473-475.

Topalidou, I., P.A. Chen, K. Cooper, S. Watanabe, E.M. Jorgensen, M. Ailion. 2017. The NCA-1 and NCA-2 Ion Channels Function Downstream of Gq and Rho to Regulate Locomotion in Caenorhabditis elegans. Genetics 206: 265-282.

German, C.L., Gudheti, M.V., Fleckenstein, A.E., E.M. Jorgensen. 2017. Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy. Methods Mol Biol, 1663:153-162.

Geisler, F., H. Gerhardus, K. Carberry, W. Davis, E M. Jorgensen, C. Richardson, O. Bossinger and R.E. Leube. 2016. A novel function for the MAP kinase SMA-5 in intestinal tube stability. Molecular Biology of the Cell 27, p3855-3868.

Edelman, T.L.B., K. A. McCulloch, A. Barr, C. Frøkjær-Jensen, E.M. Jorgensen and A. E. Rougvie. 2016. Analysis of a lin-42/periodn null allele implicates all three isoforms in regulation of Caenorhabditis elegans molting and developmental timing. G3 6:4077-4086.

Hess, J., M. Patra, L. Rangasamy, S. Konatschnig, O. Blacque, A. Jabbar, P. Mac, E.M. Jorgensen, R.B. Gasser, G. Gasser. 2016. Organometallic derivatization of the nematocidal drug monepantel leads to promising antiparasitic drug candidates. Chemistry 22, 16602-16612.

Bend, E.G.,Y.Si, D.A. Stevenson, P. Bayrak-Toydemir, T.M. Newcomb, E.M. Jorgensen, K.J. Swoboda. 2016, NALCN channelopathies: Distinguishing gain-of-function and loss-of-function mutations. Neurology 87, p1131-1139.

Wheeler, B.S., E. Anderson, C. Frøkjær-Jensen, Q. Bian, E.M. Jorgensen, B.J. Meyer. 2016. Chromosome-wide mechanisms to decouple gene expression from gene dose during sex-chromosome evolution. eLife 5:e17365. DOI: 10.7554/eLife.17365.

Frøkjær-Jensen, C., N. Jain, L. Hansen, M.W. Davis, Y. Li, D. Zhao, K. Rebora, J.R. Millet, X. Liu, S.K. Kim, D. Dupuy, E.M. Jorgensen*, and A.Z. Fire*. 2016. An Abundant class of non-coding DNA can prevent stochastic gene silencing in the C. elegans germline. Cell 166, p343-357. (*co-corresponding authors).

Li, P., S. Merrill, E.M. Jorgensen, and K. Shen. 2016. Two clathrin adaptor protein complexes instruct axon-dendrite polarity. Neuron 90, p564-580.

Schwartz, M.L., E.M. Jorgensen. 2016. SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in C. elegans. Genetics 202, p. 1277-1288. doi 10.1534/genetics.115.184275.

Alqadah, A., Y.-W. Hsieh, J.A. Schumacher, X. Wang, S.A. Merrill, G. Millington, B. Bayne, E.M. Jorgensen, C.-F. Chuang. 2016. SLO BK potassium channels couple gap junctions to inhibition of calcium signaling in olfactory neuron diversification. PLoS Genetics, 12, e1005654-e1005654.

Jobson, M.A., C.M. Valdez, J. Gardner, L.R. Garcia, E.M. Jorgensen, and A.A. Beg. 2015. Spillover transmission is regulated by the excitatory GABA receptor LGC-35 in C. elegans. J. Neurosci. 35, p2803-2816.

Watanabe, S., T. Trimbuch, M. Camacho-Pérez, B.R. Rost, B. Brokowski, B. Söhl-Kielczynski, A. Felies, M.W. Davis, C. Rosenmund, and E.M. Jorgensen. 2014. Clathrin regenerates synaptic vesicles from endosomes. Nature 515, p228-233.

Hollopeter, G.H., J.J. Lange, Y. Zhang, T.N. Vu, M. Gu, M. Ailion, E.J. Lambie, B.D. Slaughter, J.R. Unruh, L. Florens, and E.M. Jorgensen. 2014. The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex. eLife 3, 03648. doi:10.7554/eLife

Jorgensen, E.M. 2014. Animal evolution: looking for the first nervous system. Current Biology 24, pR655-R658. Review.

Liu Q, Frerck MJ, Holman HA, Jorgensen EM, Rabbitt RD. 2014. Exciting cell membranes with a blustering heat shock. Biophys J. 106(8):1570–1577. PMCID: PMC4008823

Frøkjær-Jensen, C., M.W. Davis, M. Sarov, J. Taylor, S. Flibotte, M. LaBella, A. Pozniakovsky, D.G Moerman, E.M. Jorgensen. 2014. Random and targeted integration of transgenes in C. elegans using a modified Mos1 transposon. Nature Methods 11, p529-534

Ailion, M., M. Hannemann, S. Dalton, A. Pappas, S.Watanabe, J. Hegermann, H.F. Han, M. Gu, K. Schuske, P. Hullett, S. Eimer, and E.M. Jorgensen. 2014. A Rab2 Effector Complex Mediates Dense-core Vesicle Maturation. Neuron 82, p167-180.

Rawson, R.L., L. Yam, R.M. Weimer, E.G. Bend, E. Hartwieg, H.R. Horvitz, S.G. Clark, E.M. Jorgensen. 2014. Axons degenerate in the absence of mitochondria. Curr Biol 24, p760-765.

Nix, P. M. Hammarlund, L. Hauth, M. Lachnit, E.M. Jorgensen, and M. Bastiani. 2014. Axon regeneration genes identified by RNAi screening in C. elegans. J. Neurosci. 34, p629-645.

Kavalali, E., and E.M. Jorgensen. 2014. Visualizing Presynaptic Function. Nature Neuroscience 17, p10-16. Review.

Ilovitsh, T., A. Meiri, C. Ebeling, R. Menon, J.M. Gerton, E.M. Jorgensen, and Z. Zalevsky. 2013. Improved localization accuracy in stochastic super-resolution fluorescence micoscopy by K-factor Image deshadowing. Biomedical Optics Express 5, p244–258.

Watanabe, S., B. Rost., M. Camacho, M. W. Davis, B. Söhl-Kielczynski, A. Felies, C. Rosenmund and E.M. Jorgensen. 2013. Ultrafast endocytosis at mouse hippocampal synapses. Nature 504, p242-247.

Peden, A.S., P. Mac, Y.J. Fei, C. Castro, G. Jiang, K.J. Murfitt, E.A. Miska, V. Ganapathy, E.M. Jorgensen. 2013. Betaine acts on ligand-gated ion channel in the nervous system of the nematode C. elegans. Nature Neuroscience 16, 1794-1801.

Ebeling, C. and E.M. Jorgensen. 2013. Two views on light sheets. Nature Biotechnology 31, p992–993.

Watanabe, S., Q. Liu, M.W. Davis , N. Thomas, J. Richards, G. Hollopeter, M. Gu, N.B. Jorgensen and E.M. Jorgensen. 2013. Ultrafast endocytosis at the C. elegans neuromuscular junction. eLife 2, e00723.

Ritter, B., S. Murphy, M.V. Gudheti, M. Halin, J. Philie, E.M. Jorgensen, K. Gehring, and P.S. McPherson. 2013. NECAP 1 regulates AP-2 interactions to control vesicle size, number and cargo during clathrin-mediated endocytosis.PLoS Biology 11, e1001670.

Hodges, J., X. Tang, M. Landesman, J.B. Ruedas, A. Ghimire, M.V. Gudheti, J. Perrault, E.M. Jorgensen, J.M. Gerton and S. Saffarian. 2013. Asymmetric packaging of polymerases within vesicular stomatitis virus. Biochem Biophys Res Commun 440, p271-276.

Shao, Z., S. Watanabe, R. Christensen, E.M. Jorgensen and D.A. Colón-Ramos. 2013. Synapse location during growth depends on glia location. Cell 154, p337-350.

Qi, Q.B., M.D. Po, P. Mac, T. Kawano, E.M. Jorgensen, M. Zhen, Y. Jin. 2013. Hyperactivation of B-type motor neurons results in aberrant synchrony of the Caenorhabditis elegans motor circuit. J. Neurosci. 33, p5319-5325.

Gu, M., Q. Liu, S. Watanabe, L. Sun, B. Grant, and E.M. Jorgensen. 2013. AP2 hemicomplexes contribute independently to synaptic vesicle endocytosis. eLife 2, p00190

Jurrus, E.. S. Watanabe, R. J. Giuly, A. R. C. Paiva, M. Ellisman, E.M. Jorgensen, T. Tasdizen. 2013. Semi-automated neuron boundary detection and nonbranching process segmentation in electron microscopy images.Neuroinformatics 10, p1-25.

Gu, M., Q. Liu, S. Watanabe, L. Sun, G. Hollopeter, B. Grant, and E.M. Jorgensen. 2013. AP2 subunits contribute independently to synaptic vesicle endocytosis. eLife 2, 00190.

Watanabe, S., Richards, J., Hollopeter, G., Hobson, R.J., Davis, M.W., and Jorgensen, E.M. 2012. Nano-fEM: protein localization using correlative photo-activated localization microscopy and electron microscopy. Journal of Visual Experiments 3: e3995. doi: 10.3791/3995.

White, J.Q. and E.M. Jorgensen. 2012. Sensation in a single neuron pair represses male behavior in hermaphrodites. Neuron 75, p593-600.

Mullen, G.P., K.M. Grundahl, M. Gu, S. Watanabe, R.J. Hobson, J.A. Crowell, J.R. McManus, E.A. Mathews, E.M. Jorgensen, and J.B. Rand. 2012. UNC-41/Stonin functions with AP2 to recycle synaptic vesicles in Caenorhabditis elegans. PLoS ONE 7:e40095.

Jurrus, E.. S. Watanabe, R. J. Giuly, A. R. C. Paiva, M. Ellisman, E.M. Jorgensen, T. Tasdizen. 2012. Semi-automated cell boundary detection and nonbranching process segmentation in electron microscopy images. Neuroinformatics 10, p1-25.

Carberry K, Wiesenfahrt T, Geisler F, Stöcker S, Gerhardus H, Überbach D, Davis W, Jorgensen E.M., Leube RE, Bossinger O. 2012. The novel intestinal filament organizer IFO-1 contributes to epithelial integrity in concert with ERM-1 and DLG-1. Development 139, p1851-1862.

Ernstrom, G., R.M. Weimer, D. Greenstein and E.M. Jorgensen. 2012. The V-ATPase subunit B is required for synaptic vesicle acidification and locomotion in Caenorhabditis elegans. Genetics 191, p461-475.

Watanabe, S., G. Oikonomou, S. Shaham and E.M. Jorgensen. 2011. Molecular Architecture of a Sensory Ending Using Correlative Fluorescence and Electron Microscopy. Microscopy and Microanalysis 17 (Suppl. 2) , 362-363. doi:10.1017/S1431927611002686.

Frøkjær-Jensen, C., M.W. Davis, M. Ailion, E.M. Jorgensen. 2012. Improved Mos1-mediated transgenesis in C. elegans. Nature Methods 9, p117-118.

Watanabe, S., C. Ebeling, G. Oikonomou, S. Shaham, J. Gerton and E.M. Jorgensen. 2011. Three-Dimensional Reconstruction of Actin in a Sensory Glial Cell using Bi-Plane PALM. Microscopy and Microanalysis 17 (Suppl. 2) , p16-17. doi:10.1017/S143192761100095X.

Oikonomou, G., E.A. Perens, Y. Lu, S. Watanabe, E.M. Jorgensen and S. Shaham. 2011. Opposing activities of LIT-1/NLK and DAF-6/Patched-related direct sensory compartment morphogenesis in C. elegans. PLoS Biology 9, e1001121.

Phirke, Prasad, E. Efimenko, S. Mohan, J. Burghoorn, F. Crona, M.W. Bakhoum, M. Trieb, K. Schuske, E. M. Jorgensen, B. P Piasecki, M. R Leroux, and P. Swoboda. 2011. Transcriptional profiling of C. elegans DAF-19 uncovers a ciliary base-associated protein and a CDK/CCRK/LF2p-related kinase required for intraflagellar transport. Dev Biol. 357, p235-247.

Frøkjær-Jensen, C., M.W. Davis, M. Ailion, E.M. Jorgensen. 2012. Improved Mos1-mediated transgenesis in C. elegans. Nature Methods 9, 117-118.

Oikonomou G, Perens EA, Lu Y, Watanabe S, Jorgensen EM, Shaham S. Opposing Activities of LIT-1/NLK and DAF-6/Patched-Related Direct Sensory Compartment Morphogenesis in C. elegans.PLoS Biol. 2011 Aug;9(8):e1001121.

Phirke P, Efimenko E, Mohan S, Burghoorn J, Crona F, Bakhoum MW, Trieb M, Schuske K, Jorgensen EM, Piasecki BP, Leroux MR, Swoboda P. Transcriptional profiling of C. elegans DAF-19 uncovers a ciliary base-associated protein and a CDK/CCRK/LF2p-related kinase required for intraflagellar transport. Dev Biol. 2011 Sep 1;357(1):235-47.

Batchelder EL, Hollopeter G, Campillo C, Mezanges X, Jorgensen EM, Nassoy P, Sens P, Plastino J. Membrane tension regulates motility by controlling lamellipodium organization. Proc Natl Acad Sci U S A. 2011 Jul 12

Zhang H, Constantine R, Vorobiev S, Chen Y, Seetharaman J, Huang YJ, Xiao R, Montelione GT, Gerstner CD, Davis MW, Inana G, Whitby FG, Jorgensen EM, Hill CP, Tong L, Baehr W. UNC119 is required for G protein trafficking in sensory neurons. Nat Neurosci. 2011 Jun 5

Zeiser E, Frøkjær-Jensen C, Jorgensen E, Ahringer J. MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline. PLoS One. 2011;6(5):e20082.

Park M, Watanabe S, Poon VY, Ou CY, Jorgensen EM, Shen K. CYY-1/cyclin Y and CDK-5 differentially regulate synapse elimination and formation for rewiring neural circuits. Neuron. 2011 May 26

Hobson RJ, Liu Q, Watanabe S, Jorgensen EM. Complexin maintains vesicles in the primed state in C. elegans. Curr Biol. 2011 Jan 25;21(2):106-13.

Watanabe S., A. Punge , G. Hollopeter , K.I. Willig, R.J. Hobson , M.W. Davis , S.W. Hell , and E.M. Jorgensen. 2011. Protein localization in electron micrographs using fluorescence nanoscopy. Nature Methods 8, p80-84.

Rathore S.S., E.G. Bend, H. Yu, M. Hammarlund, E.M. Jorgensen and J. Shen. 2010. Syntaxin N-peptide motif is an initiation factor for the assembly of the SNARE-Sec1/Mun18 membrane fusion complex. Proc. Natl. Acad. Sci. 107, p22399-22406.

Jurrus, E., A.R. Paiva, S. Watanabe, J.R. Anderson, B.W. Jones, R.T. Whitaker, E.M. Jorgensen, R.E. Marc, and T Tasdizen. 2010. Detection of neuron membranes in electron microscopy images using serial neural network architecture. Medical Image Analysis 14, 770-783.

Frøkjær-Jensen, C., M.W. Davis, G. Hollopeter, J. Taylor, T. Harris, P. Nix, R. Lofgren, M. Bastiani, D.G. Moerman, E.M. Jorgensen. 2010. Targeted gene deletions in C. elegans. Nature Methods 7:451-453.

Ou, C.Y., V.Y. Poon, C. Maeder, S. Watanabe, E. Lehrman, A.K.Y. Fu, M. Park, W.Y. Fu, E.M. Jorgensen, N.Y. Ip, and K. Shen. 2010. Two cyclin-dependent kinase pathways are essential for polarized trafficking of presynaptic components. Cell141:846-858.

Jospin, M., Y.B. Qi, T. Stawicki, T. Boulin, K.R. Schuske, H.R. Horvitz, J.L. Bessereau, E.M. Jorgensen and Y. Jin. 2009. A neuronal acetylcholine receptor regulates the balance of muscle excitation and inhibition in C. elegans. PLoS Biology 7, e1000265.

Frøkjær-Jensen, C., M.W. and E.M. Jorgensen. 2009. Calcium: an insignificant thing.  Nature Neuroscience 12, 1213-1214. Review.

Liu, Q., G. Hollopeter and E.M. Jorgensen. 2009. Graded synaptic transmission at the Caenorhabditis elegans neuromuscular junction. Proc Natl Acad Sci. 106(26):10823-3.

Sato K., Ernstrom G.G., Watanabe S., Weimer R.M., Chen C.H., Sato M., Siddiqui A., Jorgensen E.M., Grant B.D. 2009. Differential requirements for clathrin in receptor-mediated endocytosis and maintenance of synaptic vesicle pools. Proc Natl Acad Sci USA 106:1139-1144..

Gu, M., K. Schuske, S.i Watanabe, Q. Liu, P. Baum, G. Garriga, and E.M. Jorgensen. 2008. mu2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans. J. Cell Biol. 183, 881-892.

Frøkjær-Jensen, C., M.W. Davis, C.E. Hopkins, B. Newmann, J.M. Thummel, S-P. Olesen, M. Grunnet and E.M. Jorgensen. 2008. Single copy insertion of transgenes in C. elegans. Nature Genetics 40, 1375-1383.

Hammarlund, M., P. Nix, L. Hauth, E.M. Jorgensen, and M..J. Bastiani. 2009. Axon regeneration requires a conserved MAP kinase pathway. Science 323, 802-806.

Palfreyman, M., and E.M. Jorgensen. 2009. In vivo analysis of membrane fusion. In Encyclopedia of Life Sciences, John Wiley & Sons. Chichester http://www.els.net/ [DOI: 10.1002/ 9780470015902.a0020891]. Review.

Palfreyman, M., and E.M. Jorgensen. 2008. Roles of SNARE proteins in synaptic vesicle fusion. In Molecular Mechanisms of Neurotransmitter Release, Humana Press, p35-59. Review

Davis, M.W., J. Morton, D. Carroll and E.M. Jorgensen. 2008. Gene activation using FLP recombinase in C. elegans. PLoS Genetics 4(3) e1000028.

Robert, V.J., M.W. Davis, E.M. Jorgensen and J-L Bessereau. Gene conversion and end-joining repair double-strand breaks in the C. elegans germline. Genetics 180, 673 - 679.

Hammarlund, M., S. Watanabe, K. Schuske, and E.M. Jorgensen. 2008. CAPS and syntaxin dock dense core vesicles to the plasma membrane in neurons. Journal of Cell Biology 180 483-491.

Pan, C.L, P. Baum, M. Gu, E. M. Jorgensen, S. Clark, and G. Garriga. 2008. C. elegans AP-2 and retromer control Wnt secretion by regulating MIG-14/Wntless.  Developmental Cell 14, 132-139.

Jung, N.,  M. Wienisch, M. Gu, J.B. Rand, S. L Müller, G. Krause, E. M. Jorgensen, J. Klingauf, V. Haucke. 2007. Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2. Journal of Cell Biology 179, 1497-1510.

Beg, A., P., G. Ernstrom, P. Nix, M.W. Davis and E.M. Jorgensen. 2008. Protons act as a transmitter for muscle contraction in C. elegans. Cell 132: 149-160.

Williams, S.L., S. Lutz, N.K. Charlie, C. Vettel, M. Ailion, C.Coco, J.J.G. Tesmer, E.M. Jorgensen, T. Wieland, and K.G. Miller.  2007.  Trio's Rho-specific GEF domain is the missing Gq effector in C. elegans. Genes and Development 21, 2731-2746.

White, J.Q., T.J. Nicholas, J. Gritton, L. Truong, E.R. Davidson and E.M. Jorgensen. 2007. The sensory circuitry for sexual attraction in C. elegans males. Current Biology 17, 1847-1857.

Peters, M. T. Teramoto, J.Q. White, K. Iwasaki and E. M. Jorgensen. A calcium wave mediated by gap junctions coordinates a rhythmic behavior in C. elegans.  2007. Current Biology 17, 1601-1608.

Jospin, M., S. Watanabe, D. Joshi, S. Young, K. Hamming, C. Thacker, T. Snutch, E.M. Jorgensen and K. Schuske. 2007. UNC-80 and the NCA ion channels contribute to endocytosis defects in synaptojanin mutants. Current Biology 17, 1595-1600.

Hammarlund, M., M. Palfreyman, S. Watanabe, S. Olsen, and E.M. Jorgensen. 2007. Open syntaxin docks synaptic vesicles. PLoS Biology 5 (8): e198, 1695-1711.

Schuske, K. M.T. Palfreyman, S. Watanabe, and E. M. Jorgensen. 2007. UNC-46, a novel protein required for trafficking of the vesicular GABA transporter. Nature Neuroscience 10, 846-853.

Palfreyman, M., and E.M. Jorgensen. 2007. PKC defends crown against Munc13. Neuron 54, 179-180. Review.

Speese, S., M. Petrie, K. Schuske, M. Ailion, K. Ann, K. Iwasaki, E.M. Jorgensen, and T.F.J. Martin. 2007. UNC-31 (CAPS) Is Required for Dense-Core Vesicle But Not Synaptic Vesicle Exocytosis in Caenorhabditis elegans. Journal of Neuroscience 27, 6150-6162.

Bednarek, E., L. Schaheen, J. Gaubatz, E.M. Jorgensen and H. Fares. 2007. The plasma membrane calcium-ATPase is required for clathrin-mediated endocytosis. Traffic 8, 543-553.

Hammarlund, M., E.M. Jorgensen, M.J. Bastiani. 2007. Axons break in animals lacking beta-spectrin. J Cell Biol. 176, 269-275.

Morton, J., M.W. Davis, E.M. Jorgensen, and D. Carroll. 2006. Induction and repair of zinc-finger nuclease-targeted double-strand breaks in C. elegans somatic cells. Proc Natl Acad Sci USA 103, 16370-16375.

Abraham, C., H. Hutter, M.T. Palfreyman, G. Spatkowski, R. Weimer, R. Windoffer, E.M. Jorgensen and R. Leube. 2006. Synaptic tetraspan vesicle membrane proteins are conserved but not needed for synaptogenesis and neuronal function in C. elegans. Proc Natl Acad Sci USA 103, 8227-8232.

Wardell, B., P.S. Marik, D. Piper, T. Rutar, E.M. Jorgensen and B.A. Bamber. 2006.  Residues in the first transmembrane domain of the Caenorhabditis elegans GABAA receptor confer sensitivity to the neurosteroid pregnenolone sulfate.  Brit. J. Pharm.  148, 162-172.

Davis*, M.W., M. Hammarlund*, T. Harrach, S. Olsen, and E.M. Jorgensen. 2005. Rapid single nucleotide polymorphism mapping in C. elegans.  BMC genomics 6, 118.

Hammarlund, M.*, M.W. Davis*, H. Nguyen, D. Dayton, and E.M. Jorgensen. 2005. Heterozygous insertions alter crossover distribution but allow crossover interference in C. elegans.  Genetics, 171: 1047-1056. Supplementary Data

  • Commentary: Hawley, R. S. (2005). Faculty of 1000. PDF

Williams, D.C., T. Boulin, A.F. Ruaud, E.M. Jorgensen, and J.L Bessereau. 2005. Characterization of Mos1-Mediated Mutagenesis in Caenorhabditis elegans: A Method for the Rapid Identification of Mutated Genes.  Genetics, 169: 1779-1785.

Bamber, B.A., J.E. Richmond, J.F. Otto and E.M. Jorgensen. 2005.  The composition of the GABA receptor at the Caenorhabditis elegans neuromuscular junction.  Br. J. Pharm. 144, 502 - 509.

Davis, M.W., A.J. Birnie, A.C. Chan, A.P. Page, and E.M. Jorgensen. 2004. A conserved metalloprotease mediates ecdysis in C. elegans.  Development 131, 6001-6008.

Jorgensen, E.M. 2004. Dopamine: should I stay or should I go now?  Nature Neuroscience 7, 1019-1021. Review.

Schuske, K., and E.M. Jorgensen, 2004. The vesicular glutamate transporter: shooting blanks.  Science 304, 1750-1752. Review.

Schuske, K., A. Beg, and E.M. Jorgensen, 2004. The GABA nervous system in Caenorhabditis elegans.  Trends in Neurosci. 27, p407-414. Review.

Rostaing, P., R.M. Weimer, E.M. Jorgensen, A. Triller and J.L Bessereau. 2004. Preservation of immunoreactivity and fine structure of C. eleganstissues using high-pressure freezing.  J. Histochemistry and Cytochemistry 52: 1–12.

Schuske, K.R., J.E. Richmond, D.S. Matthies, W.S. Davis, S. Runz, D.A. Rube, A.M. van der Bliek and E.M. Jorgensen. 2003. Endophilin is required for synaptic vesicle endocytosis by localizing synaptojanin.  Neuron 40, 749-762.

  • Commentary: Endophilin and synaptojanin hook up to promote synaptic vesicle endocytosis.  Song and K. Zinsmaier.  2003.  Neuron 40, 665-670. PDF

Beg, A., and E.M. Jorgensen. 2003. An excitatory GABA receptor. Nature Neuroscience 6, 1145-1152.

  • Commentary: Jones. 2003. An exciting GABA receptor. Nature Reviews Neuroscience PDF

  • Commentary: Goaillard, J-M., and E. Marder.  2003. Exciting guts with GABA. Nature Neuroscience 6, 1121-1122. PDF

Lesa, G., M. Palfreyman, D. H. Hall, M. T. Clandinin, C. Rudolph, E.M. Jorgensen and G. Schiavo. 2003. Long chain polyunsaturated fatty acids are required for efficient neurotransmission in C. elegans.  J. Cell Science 116, 4965-4975.

Weimer, R., J.E. Richmond, W.S. Davis, J. Gritton, G. Hadwiger, M. Nonet and E.M. Jorgensen. 2003. Defects in synaptic vesicle docking in unc-18 mutants. Nature Neuroscience 6, 1023-1030.

Weimer, R., and E.M. Jorgensen. 2003. Controversies in synaptic vesicle exocytosis.  J. Cell Sci. 116, 3661-3666.   Review. 

Bamber, B.A.; R.E. Twyman, and E.M. Jorgensen. 2003. Pharmacological characterization of the homomeric and heteromeric UNC-49 GABA receptors in C. elegans  British Journal Pharmacology, 138, 883-893.

Corsi A.K. T.M., Brodigan, E.M. Jorgensen, M. Krause. 2002. Characterization of a dominant negative C. elegans Twist mutant protein with implications for human Saethre-Chotzen syndrome. Development 129: 2761-2772.

Jorgensen, E.M., and S.E. Mango. 2002. The art and design of genetic screens: Caenorhabditis elegans. Nature Review Genetics 3, 356-369.

Halevi, S., J. McKay, M. Palfreyman, L. Yassin, M. Eshel, E. Jorgensen, and M. Treinin. 2002. The C. elegans ric-3 gene is required for maturation of nicotinic acetylcholine receptors.  EMBO J. 21, 1012-1020.

Knobel, K., W. Davis and E.M. Jorgensen, and M. Bastiani. 2001. UNC-119 suppresses axon branching in C. elegans Development  128: 4079-4092.

Koushika, S., J. E. Richmond, G. Hadwiger, R. Weimer, E. M. Jorgensen and M. L. Nonet. 2001. A post-docking role for active zone protein Rim.  Nature Neuroscience 4, 997-1005.

Bessereau, J.L., A. Wright, D.C. Williams, K. Schuske, M.W. Davis and E.M. Jorgensen.  2001. Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line. Nature 413, 70-74.

Harris, T.W., K. Schuske, and E.M. Jorgensen. 2001. Synaptic vesicle endocytosis in C. elegans. Traffic 2, 597-605.  Review.

Richmond, J.E., R.M. Weimer, and E.M. Jorgensen. 2001. An open form of syntaxin bypasses the requirement for UNC-13 in vesicle priming.  Nature 412, 338-341.

Yook, K.J., S.R. Proulx and E.M. Jorgensen. 2001. Rules of nonallelic noncomplementation at the synapse in Caenorhabditis elegans.  Genetics 158, 209-220.

Harris, T.W., E. Hartwieg, H.R. Horvitz, and E.M. Jorgensen. 2000. Mutations in synaptojanin disrupt vesicle recycling. J Cell Biol 150, 589-600.

Hammarlund, M., W.S. Davis and E.M. Jorgensen. 2000. Mutations in b-spectrin disrupt axon outgrowth and sarcomere structure.  J. Cell Biol. 149, 931-942.

  • Commentary: A protein accumulator.  Pinder, J.C. and A.J. Baines.  2000. Nature 406, 253-254. PDF

 Richmond, J.E., W.S. Davis, and E.M. Jorgensen. 1999. UNC-13 is required for synaptic vesicle fusion in C. elegans.  Nature Neuroscience 2, 959-964.

Dal Santo, P., M.A. Logan, A.D. Chisholm and E.M. Jorgensen. 1999.The inositol trisphosphate receptor regulates a 50 second behavioral rhythm in C. elegans.  Cell 98, 757-767.

Knobel, K., E.M. Jorgensen and M. Bastiani. 1999. Growth cones stall and collapse during C. elegans development. Development 126, 4489-4498.

Richmond, J.E., and E.M. Jorgensen. 1999. One GABA and two acetylcholine receptors function at the C. elegans neuromuscular junction. Nature Neuroscience 2, 791-797.

Nonet, M., A.M. Holgado, F. Brewer, C.J. Serpe, B.A. Norbeck, J. Holleran, L. Wei, E. Hartweig, E.M. Jorgensen, and A. Alfonso. 1999. UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and protein composition of synaptic vesicles. Mol. Biol. Cell 10, 2343-2360.

Bamber, B., Beg, A., Twyman, R. and E.M. Jorgensen. 1999. The Caenorhabditis elegans unc-49 locus encodes multiple subunits of a heteromultimeric GABA receptor  J. Neuroscience 19, 5348-5359.

Jin, Y., Jorgensen, E., Hartwieg, E. and H.R. Horvitz. 1999. The C. elegans gene unc-25 encodes glutamic acid decarboxylase and is required for synaptic transmission but not synaptic development. J. Neuroscience 19, 539-548.

Chalfie, M., Jorgensen, E.M.. 1998. C. elegans neuroscience: Genetics to genome. Trends Genet. 14:506-512.

Gilbert, S.F., and E.M. Jorgensen. 1998. Wormwholes: a commentary on K.F. Schaffner's 'Genes, behavior and developmental emergentism'. Philosophy of Science 65, 259-266.

McIntire, S.L., R.J. Reimer, K. Schuske, R.H. Edwards and E.M. Jorgensen. 1997. Identification and characterization of the vesicular GABA transporter. Nature 389, 870-876.

Nonet, M.L., J.E. Staunton, M. Kilgard, E. Hartwieg, T. Fergestad, H.R. Horvitz, E.M. Jorgensen, B.J. Meyer. 1997. C. elegans rab-3 mutant synapses exhibit impaired function and are partially depleted of vesicles. J. Neurosci. 17: 8061-8073.

Jorgensen, E.M., Hartwieg, E., Yook, K., and Horvitz, H.R. 1996. Genetic screens for synaptic function mutants in the nematode C. elegans.  In Basic Neuroscience in Invertebrates. Tenth Tokyo Metropolitan Institute for Neuroscience International Symposium. p. 217-226.

 

Jorgensen, E.M, E. Hartwieg, K. Schuske, M.L. Nonet, Y. Jin and H.R. Horvitz. 1995. Defective recycling of synaptic vesicles in synaptotagmin mutants of Caenorhabditis elegans. Nature 378: 196-199.

Jorgensen, E.M. and M. Nonet.  1995.  The C. elegans neuromuscular junction.  Seminars in Developmental Biology  6, 207-220.

McIntire, S., E.M. Jorgensen, and H.R. Horvitz. 1993. Genes required for GABA function in the nematode Caenorhabditis elegans. Nature 364: 334-337.

McIntire, S., E.M. Jorgensen, J. Kaplan and H.R. Horvitz. 1993. The GABAergic nervous system of Caenorhabditis elegans. Nature 364: 337-341.

Riley, G.R., E.M. Jorgensen, R.K. Baker, and R. Garber. 1991. Positive and negative control of the Antennapedia promoter P2.  Development Sup. 1: 177-185.

Jorgensen, E.M., and R. Garber. 1987. Function and misfunction of the two promoters of the Antennapedia gene in Drosophila. Genes and Development 1: 544-555.

Stroeher, V., E.M. Jorgensen, and R. Garber. 1986. Multiple transcripts from the Antennapedia gene of Drosophila melanogaster. Mol. Cell. Biol. 6: 4667-4675.

 

book chapters

Hobson, R.J., K. J. Yook and E. M. Jorgensen. 2017. Genetics of Neurotransmitter Release in Caenorhabditis elegans. In Reference Module in Life Sciences, Elsevier, ISBN: 978-0-12-809633-8.

Watanabe, S., M. Lehmann, E. Hujber, R.D. Fetter, J. Richards, B. Söhl-Kielczynski, A. Felies, C. Rosenmund, J. Schmoranzer, and E.M. Jorgensen. 2014. Nanometer-resolution fluorescence electron microscopy (nano-fEM) in cultured cells. In Methods in Molecular Biology: Electron Microscopy, Ed. J. Kuo, 1117: 503-526.

LaBella, M.L., S. Sigrist, and E.M. Jorgensen. 2013. Putting genetics into optogenetics: knocking out proteins with light. In Optogenetics. Ed. Hegemann and Sigrist. Dahlem proceedings, De Guyer Publications. p 79-90. Review.

Hobson, R.J., K. Yook and E.M. Jorgensen. 2013. Neurogenetics of Neurotransmitter Release in Caenorhabditis elegans neurotransmitter release. In Brenner’s Encyclopedia of Genetics, 2nd Edition, Ed. S. Maloy, K. Hughes, Elsevier Science, San Diego, pp. 46-51.

Watanabe, S., and E.M. Jorgensen. 2012. Visualizing proteins in electron micrographs at nanometer resolution. In Correlative Light and Electron Microscopy, Ed. T. Müller-Reichert and P. Verkade. Methods in Cell Biology 111, p283-306.

Jorgensen, E.M.  2005.  GABA. WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.14.1, http://www.wormbook.org.  Book Chapter.

Yook, K. and E.M. Jorgensen. 2000. Genetics of the Caenorhabditis elegans nervous system.  In Encyclopedia of Genetics.  Ed. S. Brenner, J. Miller, Academic Press, New York. pp. 1309-1314.

Jorgensen, E.M., and C. Rankin. 1997. Neural Plasticity.  In:C. elegans II.  Ed. D. Riddle, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.  pp. 769-790.